Exsil AVANTI BDS Kolonlar 3.5 ve 10um Seçenekler
Avanti BDS
Alternative to Hypersil BDS range of phases.
Extended range including 3 μm
Pore Size: 145 (+/-5) Å,
Pore Volume: 0.68 ml/g,
Surface Area: 186 m2/g
Particle sizes: Available in 1.5, 3, 5 and 10 particle sizes.
Phases: Silica, C8, CN, C18 and Quattro.
The Avanti BDS range was developed to meet the need for a
range of base deactivated reversed phases based on a Type B silica which are
less retentive than the standard 80 and 100 ranges and provide a closer
match to current established commercial BDS 120 - 150Å media. C18, C8
and CN phases are available in industry standard 3 and 5 μm particle sizes
and are fully end capped.
A polar embedded phase that gives excellent peak shape and low retention for difficult bases at pH 7. Acids, bases and chelates are readily eluted as sharp peaks at pH 2 using TFA.
Universal Polar Embedded Alkylamide
Exmere’s first polar embedded phase, the Avanti Quattro,
gives excellent performance for all compounds at low pH and exceptional
performance for bases at neutral pH. To compliment its performance
envelope, we have produced an alkylamide phase based on our Avanti BDS.
The Alkam phase utilises our highly purified, base deactivated,
145Å Avanti BDS which is carefully bonded with a stable alkyl amide phase of
medium chainlength using decanoic acid. This results in a phase which shows
reduced base retention compared with an alkyl bonded phase on the same silica.
Peak shape for bases is also improved over the alkyl phase while the problem of
tailing with acids which is sometimes encountered with amide phases, is
overcome by the careful bonding procedure.
The attached selection of chromatograms shows the excellent
peak shape of acids, bases and chelates at low pH and the maintenance of good
peak shape for bases at neutral pH.
Phase stability appears good and it is even capable of being
operated at pH 12 for short periods with minimal change as is shown
in the attached chromatograms. Longer periods at this pH should be
avoided. The phase resists phase collapse at low organic content and
allows its use with 100% aqueous eluents.
Compared with the Avanti Quattro, the retention of bases is higher and there is a difference in selectivity e.g. at pH 7, Nortriptyline elutes before Doxepin on the Quattro but after on the Alkam.
AVANTI BDS FLUORO
Phase: Fluoroalkyl, end capped
Particle size 1.5, 3, 5, 7 and 10µm
Base Silica: Avanti BDS
Fluorine containing phases have attracted some interest due
to the alternative selectivity they show compared with alkyl phases. In
addition to hydrophobicity which they share with alkyl phases, they also show
oleophobicity and increased interactions with fluorine containing species.
Although these differences are not always pronounced, they can provide a useful
alternative selectivity.
Perfluroaryl phases have been marketed for some time but they have a reputation for instability due to the perfluoroaryl being a good leaving group in terms of SN2 reaction.
Our approach has been to use the more stable Tridecafluorooctyl ligand attached to our Avanti BDS and end capped. A noncapped version can be supplied if required.
The attached chromatograms show the increased retention of a perflurobenzene and the reduced retention of hydrocarbons when compared to the Avanti C8 BDS phase.
The BDS base also allows the phase to show good peak shape
with bases under the usual low pH conditions but with longer retention than the
C8 phase.
Due to the low surface energy of the packings, conventional slurry solvents can lead to aggregation. We have found dichloromethane to be a suitable slurry solvent for packing